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Dr. Kathryn Moore

Event Start: Mar 07, 2017 1:00 pm
Location: Botterell Hall, Room B139


Dr. Kathryn Moore
Jean and David Blechman Professor of Cardiology, Department of Medicine
Professor, Department of Cell Biology
 
NYU School of Medicine 

Long non-coding RNA CHROME functions as a competing endogenous RNA to regulate cholesterol homeostasis


Thousands of long non-coding RNAs (lncRNAs) have been identified in the human genome,many of which are not conserved in lower mammals. The majority of these lncRNAs remain functionally uncharacterized and may have important implications in human physiology and disease. We have identified a primate-specific lncRNA, CHROME, which is increased in the plasma and atherosclerotic plaques of individuals with coronary artery disease compared to healthy controls. Using gain- and loss-of-function approaches, we show that CHROME functions as a competing endogenous RNA of microRNAs (miRNAs) that repress cellular cholesterol efflux and plasma HDL levels, and regulates the concentration and biological functions of these miRNAs. In hepatocytes and macrophages, CHROME promotes the efflux of excess cholesterol, and this atheroprotective function of CHROME is abrogated upon mutation of its miRNA binding domains. Finally, we find that hepatic levels of CHROME are positively correlated with plasma levels of HDL cholesterol in healthy individuals. Collectively, our findings identify CHROME as a central component of the non-coding RNA circuitry controlling cholesterol homeostasis in humans.



Tuesday, March 7, 2017
1:00 - 2:00pm
Botterell Hall, Room B139