My clinical interests focus on the chronic idiopathic inflammatory bowel diseases such as Ulcerative Colitis and Crohn's disease. Our research interests focus on the epithelial layer of the intestine and the identification and study of epithelial cytoprotective genes and signaling pathways. The long-term goal our the lab is to characterize novel epithelial mechanisms of cellular defence in order to exploit these endogenous protective pathways and apply this new knowledge to counteract key pathologic processes characteristic of intestinal inflammation seen in Inflammatory Bowel Disease. Our principal focus is the cytokine Interleukin-11. The role of IL-11 in protecting the vulnerable proliferative compartment of the epithelial crypt from injury through the antagonism of apoptosis is a principal focus of currently funded projects. The importance of cytoprotection and signaling by IL-11 in the intestinal epithelium is be tested in various models of intestinal inflammation with a focus on how IL-11 facilitates recovery of the epithelium and stimulates healing. Cognizant of how GI cancers exploit survival signaling, we have a shared interest in colon cancer that develops in the setting of chronic inflammation of the colon.
1. Ropeleski M. J., Tang J., Musch M. W. and Chang E. B. Interleukin-11 induced heat shock protein 25 confers intestinal epithelial specific cytoprotection from oxidant stress. Gastroenterology 124: 1358-1368, 2003
2. Kojima K, Musch MW, Ropeleski MJ, Boone DL, Ma A, Chang EB. E. coli Lipopolysaccharide Induces Heat Shock Protein 25 in Intestinal Epithelial Cells Through MAP Kinase Activation. Am J Physiol Gastrointest Liver Physiol. 286:G645-52, 2004
3. Elaine O. Petrof, Keishi Kojima, Mark J. Ropeleski, Mark W. Musch, Yun Tao, Claudio De Simone, and Eugene B. Chang. Probiotics Inhibit NF-kB Activity and Induce Heat Shock Proteins in Colonic Epithelial Cells Through Proteasome Inhibition. Gastroenterology 127:1474-87, 2004
4. Mark J. Ropeleski, Jacob R. Riehm, Kathy Baer, Mark W. Musch and Eugene B. Chang. Anti-Apoptotic Effects of L-glutamine Mediated Transcriptional Modulation of the Heat Shock Protein 72 during Heat Shock. Gastroenterology 129: 170-184, 2005
5. Kuljit Parhar, Kathy A. Baer, Kristy Parker, and Mark J. Ropeleski. Short-chain fatty acid mediated phosphorylation of heat shock protein 25: effects on camptothecin-induced apoptosis. Am J Physiol Gastrointest Liver Physiol. 291:G178-88, 2006
6. Kittiporn Phanvijhitsiri, Mark W. Musch, Mark J Ropeleski, and Eugene Chang. Heat-Induction of Heat Shock Protein 25 Requires Cellular Glutamine in Intestinal Epithelial Cells. AJP: Cell Physiology 291:C290-9, 2006