The Incucyte is a plate and flask imaging system which is housed within a regular cell culture incubator and hence the cells are imaged under true physiological culture conditions, over an extended time. During the time the cells are in the imager, the collected images can be reviewed remotely from any computer on the network.
The system currently has 10x and 20x lenses, and adaptors for a wide variety of culture plates from various manufacturers. The images are available in phase contrast and 2 fluorescence channels, green (525nm, GFP, FITC Al488), and red (575nm, RFP, DSred, PI, Cy3). In addition, there is 96well scratching module for wound healing assays, ensuring an equal and even "wound" in each well and allowing for more objective measurement. Collated images are compiled typically as a time-lapse video.
The software which runs the system and analyzes the data is user friendly and set up so that assessment of confluency in cells, rate of wound healing or (random or chemotactic) migration, clustering or colony growth, spheroid formation and fluorescence expression are easily measurable. With the addition of specific dyes and probes, apoptosis and cytotoxicity measurements are attainable in relevant cell growth time with no need for reliance on specific "snapshot" time points which may be suboptimal for a given treatment.
Fees
|
Service |
Cost |
|
Instrument use (multiple users) |
$20/plate/day |
|
Innstrument use (single user) |
$80/day |
Booking
Booking Calendar: Book Here
Location
Botterell Hall, Room 316
Contact
Training and support staff (Mon-Fri 8:30 AM-4:30 PM):
Zhilin Chen (zc@queensu.ca; ext. 78238) & Xiaohu Yan (yanx@queensu.ca; ext 78265)