My lab studies tyrosine kinase signaling pathways that regulate cell motility, tissue invasion and inflammation. We currently have projects focusing on mast cells, that produce a wide array of mediators that can remodel tissues during immune responses and regulate tumour progression. We also study contributions of F-BAR proteins in metastatic cancers using cell and mouse models.
Website: Cancer Research Institute
Cancer metastasis pathways
My research program is investigating paracrine signaling pathways linked to mast cell activation, systemic mastocytosis and cancer metastasis. Of particular interest are discovering the molecular mechanisms controlling cell motility and invasion in mast cells and cancer cells. We employ RNA interference, cell biology, transgenic mouse models, and tumor xenografting in the course of these studies. We have research ongoing to study functions of kinases and adaptor proteins targeted to membranes via their F-BAR domains in mast cells and cancer cell models. These projects will help define how F-BAR proteins contribute to the dynamic control of membranes via regulating actin assembly and other pathways.
Biosketch: My training commenced at Queen's University (B.Sc.Honours Biochemisty, 1993, with distinction), and continued as a graduate student in Dr. Sonenberg’s lab at McGill University where I studied regulation of mRNA translation (Ph.D, 1998, McLaughlin Thesis award). I returned to Queen’s University for a post-doctoral fellowship with Dr. Peter Greer in the Cancer Research Labs ('98-02; CIHR PDF and Senior Research Fellowship), where I helped develop and characterize transgenic mice lacking Fer protein-tyrosine kinase activity. Studies of Fer-deficient mice have provided new insights into the role of Fer kinase in inflammation, growth factor signaling in fibroblasts, and regulation of mast cell activation and migration. I was appointed to a faculty position in Biochemistry at Queen’s in July 2002 (CIHR New Investigator award) and have mentored 13 former trainees(4 PDF, 3 PhD, 6 MSc). The lab currently consists of 1 PDF, 3 PhD students, 2 MSc students, and a research technician.
Selected Publications (since 2012)
55 T Baldassarre, P Truesdell and AW Craig (2017) Endophilin A2 promotes HER2 internalization and blockade by Trastuzumab in HER2-positive breast cancer, Breast Cancer Research, Oct 3;19(1):110.
54 Binbing Ling, Kathleen Watt, Sunandan Banerjee, Daniel Newsted, Peter Truesdell, Jarrett Adams, Sachdev Sidhu, and Andrew W Craig (2017) A novel immunotherapy targeting MMP-14 limits hypoxia, immune suppression and metastasis in triple-negative breast cancer models, Oncotarget, 8(35):58372-58385.
53 Katrina Au, Nichole Peterson, Peter Truesdell, Gillian Reid-Schachter, Runhan Ren, Kasra Khalaj, Julie-Ann Francis, Charles H Graham, Andrew W Craig, Madhuri Koti (2017) CXCL10 alters the tumour immune microenvironment and disease progression in a syngeneic murine model of high-grade serous ovarian cancer, Gynecol Oncol, 145:436-445.
52 Au KK, Le Page C, Ren R, Meunier L, Clément I, Tyrishkin K, Peterson N, Kendall-Dupont J, Childs T, Francis JA, Graham CH, Craig AW, Squire JA, Mes-Masson AM, Koti M (2016) STAT1 associated intratumoural TH1 immunity predicts chemotherapy resistance in high-grade serous ovarian cancer, J Pathol Clin Res, 2:259-270; cited by 2 papers.
51 Utpal K Mukhopadhyay, Jamaica Cass, Leda Raptis, Andrew W Craig, Véronique Bourdeau,Sonal Varma,Sandip SenGupta,Bruce E Elliott,Gerardo Ferbeyre Dataset of STAT5A status in breast cancer, Data Brief 2016 Jun; 7:490-92.
50 Utpal K Mukhopadhyay, Jamaica Cass, Leda Raptis, Andrew W Craig, Véronique Bourdeau,Sonal Varma,Sandip SenGupta,Bruce E Elliott,Gerardo Ferbeyre (2016) STAT5A is regulated by DNA damage via the tumor suppressor p53, Cytokine, 82:70-9. Cited by 4 papers; IF 2.664
49 Madison Black, Ivraym Barsoum, Peter Truesdell, Tiziana Cotechini, Margaret Petroff, Rob Siemens, Madhuri Koti, Andrew Craig and Charles Graham (2016) Activation of the PD-1/PD-L1 immune checkpoint confers chemoresistance and promotes tumor metastasis. Oncotarget 7(9):10577-67; cited by 9 papers; IF 6.359
48 N Barlow1*, Y Nasser2*, P Zhao1*, N Sharma2, R Guerrera-Alba2, LE Edgington-Mitchell, T Lieu1, NA Veldhuis1, DP Poole1, JW Conner1, AW Craig2, B Graham1, SJ Vanner2, NW Bunnett1. (2015) Demonstration of elevated levels of active cathepsin s in dextran sulfate sodium colitis using a new activatable probe, Neurogastroenterol Motil, 27:1675-1680; cited by 2 papers; IF 3.587.
47 Otto L.D. Cerqueira, Peter Truesdell, Tomas Baldassarre, Santiago A. Vilella-Arias, Kathleen Watt, Jalna Meens, Harish Chander, Cynthia A.B. Osório, Fernando A. Soares, Eduardo M. Reis* and Andrew W.B. Craig* (2015) CIP4 promotes metastasis in triple-negative breast cancer and is associated with poor patient prognosis, Oncotarget, 6:9397-408; cited 8 times; IF 6.359
46 Tomas Baldassarre, P Truesdell, K Watt, J Meens, MM Schneider, SK Sengupta, and AWB Craig (2015) Endophilin A2 Promotes Triple Negative Breast Cancer Progression and Metastasis, Mol Cancer Res, 13:1044-55; cited by 4 papers; IF 4.38.
45 P Truesdell, J Ahn, H Chander, J Meens, K Watt, X Yang, and AWB Craig (2015) CIP4 Promotes Lung Adenocarcinoma Metastasis and Is Associated with Poor Prognosis, Oncogene, 34:3527-35; cited by 9 papers; IF 8.559.
44 Harish Chander, Colin D. Brien, Peter Truesdell, Kathleen Watt, Jalna Meens, Colleen Schick, Doris Germain and Andrew W.B. Craig (2014) Toca-1 is suppressed by p53 to limit breast cancer cell invasion and tumor metastasis, Breast Cancer Research, 16:3413; cited by 5 papers; IF 5.49.
44 Namit Sharma and Andrew W Craig (2014) Leukocyte-targeting toxins as therapeutics in allergic asthma, J Leuk Biol, 97:345-6; IF 4.289.
42 Namit Sharma, Stephanie Everingham, Lifan Zeng, Zhong-Yin Zhang, Reuben Kapur, and Andrew W.B. Craig (2014) Oncogenic KIT-induced aggressive systemic mastocytosis requires SHP2/PTPN11 phosphatase for disease progression in mice Oncotarget, 5(15):6130-41; featured on the cover Aug 30 issue; cited by 6 papers; IF 6.359.
41 Namit Sharma, Stephanie Everingham, Baskar Ramdas, Reuben Kapur, and Andrew WB Craig (2014) SHP2 phosphatase promotes mast cell chemotaxis towards SCF via enhancing activation of the Lyn/Vav/Rac signaling axis, J Immunol. 192(10):4859-66; cited by 7 papers; IF 4.992.
40 Vijay Kumar, Stephanie Everingham, Christine Hall, Peter A. Greer and Andrew W.B. Craig (2014) Calpains promote neutrophil recruitment and bacterial clearance in an acute bacterial peritonitis model, Eur J Immunol., 44:831-841; featured article “In This Issue”; cited by 5 papers; IF 4.034.
39 Joseph Ahn, Peter Truesdell, Jalna Meens, Carli Kadish, Xiaolong Yang, Alexander H. Boag and Andrew W.B. Craig (2013) Fer Protein-Tyrosine Kinase Promotes Lung Adenocarcinoma Cell Invasion and Tumor Metastasis,
Mol Cancer Res, 11:952-63; cited by 13 papers; IF 4.38.
38 Maitham Khajah, Graciela Andonegui, Ronald Chan, Andrew W. Craig, Peter A. Greer & Donna-Marie McCafferty (2013) Fer Kinase Limits Neutrophil Chemotaxis towards End Target Chemoattractants,J Immunol, 190:2208-16; cited by 14 papers; IF 5362.
37 Harish Chander, Peter Truesdell, Jalna Meens and Andrew W.B. Craig (2013) Transducer of Cdc42-dependent Actin Assembly Promotes Breast Cancer Invasion and Metastasis, Oncogene 32:3080-90; featured article; cited by 12 papers; IF 8.559.
36 Andrew W.B. Craig (2012) The family infighting continues: dualling Src family kinases now linked to activating IgG receptor signalling and anaphylaxis, Frontiers in Inflammation, 3:137
35 Raghuveer Singh Mali, Peilin Ma, Li-Fan Zeng, Holly Martin, Baskar Ramdas, Yantao He, Emily Sims, Sarah Nabinger, Joydeep Gosh, Namit Sharma, Veerendra Munugalavadla, Anindya Chatterjee, Shuo Li, George Sandusky, Andrew W. Craig, Kevin D. Bunting, Gen-Sheng Feng, Rebecca J. Chan, Zhong-Yin Zhang and Reuben Kapur (2012) Role of SHP2 phosphatase in KIT induced transformation: identification of SHP2 as a druggable target in diseases involving oncogenic KIT, Blood.120:2669-78; cited by 37 papers; IF 9.898.
34 Namit Sharma, Vijay Kumar, Stephanie Everingham, Raghuveer Singh Mali, Reuben Kapur, Li-Fan Zeng, Zhong-Yin Zhang, Gen‐Sheng Feng, Karin Hartmann, Axel Roers, and Andrew W.B. Craig (2012) SH2 domain‐containing phosphatase‐2 is a critical regulator of mast cell survival and homeostasis in mice, Mol. Cell. Biol., 32:2653-2663; featured on the cover of June 2013 issue; cited by 17 papers; IF 9.06 .
33 Ester Kwok, Shengnan Zhang, Peter A. Greer, John S. Allingham and Andrew W.B. Craig (2012) FES Kinase Promotes Mast Cell Recruitment to Mammary Tumors Via the Stem Cell Factor/KIT Receptor Signaling Axis, Molecular Cancer Research, 10:881-891; featured on the cover and highlights section of July issue; cited by 9 papers IF 4.353
32 Andrew W.B. Craig (2012) FES/FER kinase signaling in hematopoetic cells and leukemias. Frontiers in Bioscience, 17:861-875; cited by 15 papers; IF 3.286 .
For a complete list of publications see: