BD FACSDiscover A8 | Biomedical and Molecular Sciences | School of Medicine

The BD FACSDiscover^™ A8 Cell Analyzer seamlessly integrates cutting-edge spectral flow cytometry with real-time imaging. By combining innovations such as BD SpectralFX^™Technology and BD CellView^™ Image Technology into a single instrument, it offers unparalleled experimental power and simplified workflows to deliver reproducible results and single-cell insights that were previously undetectable. This versatile, robust and reliable instrument comes in a 5-laser configuration (B-R-V-YG-UV) with up to 78 fluorescence detectors and 8 scatter and imaging detectors.

Benefits include:

Optimized sensitivity and resolution
Flexibility to use imaging or high-speed mode
Integrated autoloader with temperature control and sample mixing
Simplified workflow to reduce setup complexity

- BD SpectralFX^™Technology: Delivers exceptional sensitivity and resolution. Optimal hardware design, system-aware unmixing, and next-gen setup and QC based on LED calibrated gain interface provide highly accurate data consistently and easily at default gain settings.
- BD CellView^™ Image Technology: This real-time imaging technology adds a new spatial dimension to flow cytometry analysis with image parameters like Diffusivity, Max Intensity, Radial Moment and 12 other automatically generated parameters along with scatter and fluorescent images.
- Fully Integrated Autoloader: Fully integrated inside the instrument the autoloader eliminates connectivity steps and issues for sample acquisition from plates and tube racks. High throughput and reliable, it delivers the high sample utilization and low sample carryover rate, makes it easy to maintain sample integrity with temperature control and automated sample mixing and delivers walkaway automation with automated clog/bubble detection and recovery.
- BD FACSChorus^™Software: Provides an intuitive interface for step-by-step guided navigation and makes it easy to optimize, acquire and analyze data from spectral panels with visual and unique tools such as BD^® Spectral Hotspot Matrix that are built into the acquisition software.
- Fluidics: Dual-mode fluidics gives you the flexibility to get sample acquisition rates at 35,000 events/sec in high-speed mode and 12,500 events/sec in imaging mode. The instrument runs on the deionized water, eliminating the need for traditional sheath fluids for sample acquisition while volumetric acquisition and transparent merged event rate to enhance sample acquisition flexibility and accuracy.

Fees

Service	Cost
Instrument	$35/hour
Training	$50/session

Contact and Booking

Jeffrey Mewburn (mewburnj@queensu.ca, ext. 75389) and

Peter Gagolewicz (pjg@queensu.ca; 613-449-4639)

Location

Room 569, Botterell Hall