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block-system-breadcrumb-block no-title"> <div class="container"> <div class="breadcrumb-content-main"> <h2 class="page-title hide">Differential Scanning Calorimetry (DSC) </h2> <div class=""> <div class="content block-content"> <div class="breadcrumb-links"> <div class="content-inner"> <nav class="breadcrumb " role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> <span class=""> / </span> </li> <li> <a href="/node"></a> <span class=""> / </span> </li> <li> </li> <li> Differential Scanning Calorimetry (DSC) </li> <li></li> </ol> </nav> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div role="main" class="main main-page"> <div class="clearfix"></div> <div class="clearfix"></div> <div class="clearfix"></div> <div id="content" class="content content-full"> <div class="container-full"> <div class="content-main-inner"> <div id="page-main-content" class="main-content"> <div class="main-content-inner"> <div class="content-main"> <div class="region region-content"> <div id="block-gavias-financial-content" class="block block-system block-system-main-block no-title"> <div class="content block-content"> <!-- Start Display article for detail page --> <article data-history-node-id="1073" typeof="schema:WebPage" class="node node--type-page node--view-mode-full"> <header> <div class="container"> <div class="widget gsc-heading align-left style-1 small text-dark "> <h1 class="title"><span><span property="schema:name" class="field field--name-title field--type-string field--label-hidden">Differential Scanning Calorimetry (DSC)</span> </span></h1> <div class="line"><span class="one"></span><span class="second"></span><span class="three"></span></div> </div> </div> </header> <div class="node__content clearfix"> </div> <div class="gavias-content-blockbuilder"><div class="gavias-blockbuilder-content"> <div class="gbb-row-wrapper"> <div class=" gbb-row bg-size-cover" style="" > <div class="bb-inner default"> <div class="bb-container container"> <div class="row"> <div class="row-wrapper clearfix"> <div class="gsc-column col-lg-12 col-md-12 col-sm-12 col-xs-12"> <div class="column-inner bg-size-cover " > <div class="column-content-inner"> <div class="column-content "><h2>VP-DSC</h2></div> <div class="widget gsc-image " > <div class="widget-content"> <img src="https://dbms.queensu.ca/sites/dbms/files/gbb-uploads/VP-DSC_Mix-1iyhc0.png" alt="" /> </div> </div> <div class="gsc-tabs "> <div class="tabs_wrapper tabs_vertical"> <ul class="nav nav-tabs"> <li class="active"><a data-toggle="tab" href="#uid-y51oi-1"> Description </a></li> <li ><a data-toggle="tab" href="#uid-y51oi-2"> Features </a></li> <li ><a data-toggle="tab" href="#uid-y51oi-3"> Booking & Fees </a></li> </ul> <div class="tab-content"> <div id="uid-y51oi-1" class="tab-pane fade in active"><p>Differential Scanning Calorimetry (DSC) measures enthalpy (ΔH) of protein unfolding due to heat denaturation. A protein in solution is in equilibrium between the native (folded) conformation and its denatured (unfolded) state. The higher the transition midpoint (Tm) when 50% of the protein molecules are unfolded, the more stable the protein. DSC is also used to determine the change in heat capacity (ΔCp) of denaturation. DSC can elucidate the factors that contribute to the folding and stability of native proteins, including hydrophobic interactions, hydrogen bonding, conformational entropy, and the physical environment.</p> <p> </p> <p>The VP-DSC is a highly sensitive, easy-to-use differential scanning calorimeter for the study of proteins in solution without the need for labelling. It can provide fast and accurate transition midpoint (Tm) determination to understand the factors that affect the conformation and stability of a protein of interest.</p></div> <div id="uid-y51oi-2" class="tab-pane fade in "><p>The VP-DSC is controlled by VPViewer software and data analysis is performed with MicroCal-enabled Origin software. Other features include:</p> <ul> <li>Can be used with solutions that interfere with optical methods including turbid or colored solutions or particulate suspensions.</li> <li>Provides insights into mechanisms of unfolding and refolding.</li> <li>Minimal assay development needed.</li> </ul></div> <div id="uid-y51oi-3" class="tab-pane fade in "><p><strong>Fees</strong></p> <table style="width: 49.1212%;"> <tbody> <tr> <td style="width: 25.037%;"> <p><strong>Service</strong></p> </td> <td style="width: 24.963%;"> <p><strong>Cost</strong></p> </td> </tr> <tr> <td style="width: 25.037%;"> <p>Instrument </p> </td> <td style="width: 24.963%;"> <p>$5/hr up to $50/day</p> </td> </tr> <tr> <td style="width: 25.037%;"> <p>Training</p> </td> <td style="width: 24.963%;"> <p>$125/session</p> </td> </tr> </tbody> </table> <p> <strong>Contact and Booking</strong></p> <p>Xiaohu Yan (<a href="mailto:yanx@queensu.ca">yanx@queensu.ca</a>; ext. 78265) and Zhilin Chen (<a href="mailto:zc@queensu.ca">zc@queensu.ca</a>; exr. 78238)</p> <p> <strong>Location </strong></p> <p>Botterell Hall, Room 667</p> <p> </p></div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div></div></div> </article> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <footer id="footer" class="footer"> <div class="footer-inner"> <div class="footer-center"> <div class="container"> <div class="row"> <div class="footer-first col-lg-3 col-md-3 col-sm-12 col-xs-12 column"> <div class="region region-footer-first"> <div id="block-queensfooterlogo-2" class="block block-block-content block-block-contentf665d6b6-d3bf-4022-bc0c-d81efa4c3c55 no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="qu_footer_branding"></section></div> </div> </div> </div> </div> <div class="footer-second col-lg-9 col-md-9 col-sm-12 col-xs-12 column"> <div class="region region-footer-second"> <div id="block-linkfooter" class="hidden-xs hidden-sm block block-block-content block-block-contente03afdfc-b255-481b-9605-5a0e153ccacf no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="qhs_schools"></section></div> </div> </div> </div> </div> <div class="footer-third col-lg-12 col-md-12 col-sm-12 col-xs-12 column"> <div class="region region-footer-third"> <div id="block-contactinfomschq" class="block block-block-content block-block-contentfc5b443f-0626-414c-bca6-320b99b79ec2 no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="site_contact_social"> <section> <p class="title">Contact Us</p> <ul class="contact"> <li> <a href="/contact-us"><i class="fas fa-home"></i>Department of Biomedical and Molecular Sciences</a> </li> <li><i class="fas fa-phone"></i>(613)-533-2600</li> <li> <a href="mailto:dbms@queensu.ca"><i class="fas fa-envelope"></i>dbms@queensu.ca</a> </li> </ul> </section> <section> <p class="title">Follow Us</p> <ul class="social"> <li> <a href="https://twitter.com/dbmstweets" target="_blank"><i class="fa fa-twitter"></i></a> </li> </ul> </section> </section></div> </div> </div> </div> </div> <div class="footer-four col-lg-12 col-md-12 col-sm-12 col-xs-12 column"> <div class="region region-footer-four"> <div id="block-partnerhospitals" class="block block-block-content block-block-contentcff6a208-af38-48d2-a67a-b0d2c3b6d6ae no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="qhs_hospitals"></section></div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="copyright"> <div class="container"> <div class="copyright-inner"> <div class="region region-copyright"> <div id="block-gavias-financial-copyright" class="block block-block-content block-block-content6a74724d-d485-4216-a560-962e52758fde no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="qhs_copyright"></section></div> </div> </div> </div> </div> </div> </div> </footer> </div> <script src="/sites/dbms/files/js/js_8jmYR7v8lsyFnzqh2JOVdzrf1JTxmCLkupkEgLdIHSQ.js?scope=footer&delta=0&language=en&theme=gavias_financial&include=eJxtj1EOgzAMQy9U6JFQ2oYqIzQoCdO4_fhYxTT26WfZlrMoxqL7BjzC7pJl3RgdA66oFVs-JmBUt_gLAr6cqS09_ZGhwpPApsSSl7QTF9T4h41ghm7jrNIcW-m5mRq0TMCxsiTgwfw4e-vdf9gwizhqt4zpLGY4rsUvdBs0BydzytYvXOQNjcVu2Q"></script> <script src="https://www.queensu.ca/resources/libraries/react/react.production.min.js" defer=""></script> <script src="https://www.queensu.ca/resources/libraries/react/react-dom.production.min.js" defer=""></script> <script src="https://www.queensu.ca/resources/emergencyalerts-v2/emergencyalerts.js" type="module" defer=""></script> <script src="/sites/dbms/files/js/js_kr74DVM047L8YVhYPyAa0wRAlBHNhVHTxaA-bxdLVhA.js?scope=footer&delta=4&language=en&theme=gavias_financial&include=eJxtj1EOgzAMQy9U6JFQ2oYqIzQoCdO4_fhYxTT26WfZlrMoxqL7BjzC7pJl3RgdA66oFVs-JmBUt_gLAr6cqS09_ZGhwpPApsSSl7QTF9T4h41ghm7jrNIcW-m5mRq0TMCxsiTgwfw4e-vdf9gwizhqt4zpLGY4rsUvdBs0BydzytYvXOQNjcVu2Q"></script> </body> </html>