</iframe</noscript> <!-- End Google Tag Manager (noscript) --> <a href="#main-content" class="visually-hidden focusable"> Skip to main content </a> <div class="dialog-off-canvas-main-canvas" data-off-canvas-main-canvas> <div class="body-page gva-body-page"> <div id="jpreContent"> <div id="jprecontent-inner"> <div class="preloader-wrapper hidden active"> <img src="https://dbms.queensu.ca/themes/custom/gavias_financial/images/preloader/preloader-2.gif" alt="Preloader Image" /> </div> </div> </div> <header id="header" class="header-v1"> <div class="brand_header "> <div class="brand-header-inner"> <!--Queen's Utility Bar --> <div class="clearfix"> <div class="brand-header-content"> <div class="region region-brand-header"> <div id="block-emergencyalert" class="block block-emergency-alerts no-title"> <div class="content block-content"> <div id="quur-emergency-alerts"> </div> </div> </div> <div id="block-universalheader" class="block block-block-content block-block-contentbbaec8c8-893a-4c7b-9fd9-65aa7db31b19 no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="qhs_utility_bar"> <section class="inner-wrapper container"> <a class="qu-logo" href="https://www.queensu.ca/" target="_blank" rel="noopener"> <img alt="Queen's Logo" src="/themes/custom/gavias_financial/overrides/img/Queens-logo-reversed.svg" /> </a> <section class="widget-wrapper"> <nav id="sign_in_widget"> <button> Sign In <svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"> <path d="M233.4 406.6c12.5 12.5 32.8 12.5 45.3 0l192-192c12.5-12.5 12.5-32.8 0-45.3s-32.8-12.5-45.3 0L256 338.7 86.6 169.4c-12.5-12.5-32.8-12.5-45.3 0s-12.5 32.8 0 45.3l192 192z" stroke-width="4"></path> </svg> </button> <div id="sign_in_menu"> <span>University Wide</span> <ul id="university_wide_links"></ul> <span>Faculty/School Portals</span> <ul id="portal_links"></ul> </div> </nav> <section id="search_widget"> <button id="search_button"> <svg class="glass-icon" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 24 24"> <path d="M23,24c-.25,0-.51-.1-.7-.29l-7.66-7.55c-1.58,1.19-3.51,1.84-5.52,1.84C4.09,18,0,13.96,0,9S4.09,0,9.11,0s9.11,4.04,9.11,9c0,2.12-.77,4.18-2.13,5.79l7.61,7.5c.39,.39,.4,1.02,0,1.41-.2,.2-.45,.3-.71,.3ZM9.11,2c-3.92,0-7.11,3.14-7.11,7s3.19,7,7.11,7c1.83,0,3.57-.68,4.89-1.92,0,0,0,0,0,0,1.43-1.33,2.21-3.14,2.21-5.08,0-3.86-3.19-7-7.11-7Z"></path> </svg> <svg class="close-icon" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 24 24"> <path d="M12 22C6.47715 22 2 17.5228 2 12C2 6.47715 6.47715 2 12 2C17.5228 2 22 6.47715 22 12C22 17.5228 17.5228 22 12 22ZM12 20C16.4183 20 20 16.4183 20 12C20 7.58172 16.4183 4 12 4C7.58172 4 4 7.58172 4 12C4 16.4183 7.58172 20 12 20ZM12 10.5858L14.8284 7.75736L16.2426 9.17157L13.4142 12L16.2426 14.8284L14.8284 16.2426L12 13.4142L9.17157 16.2426L7.75736 14.8284L10.5858 12L7.75736 9.17157L9.17157 7.75736L12 10.5858Z"></path> </svg> </button> <form id="search_form" role="search" action="//www.queensu.ca/qtbsearch/" method="get"> <div class="inner"> <label for="QUsearch"> <svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 24 24"> <path d="M23,24c-.25,0-.51-.1-.7-.29l-7.66-7.55c-1.58,1.19-3.51,1.84-5.52,1.84C4.09,18,0,13.96,0,9S4.09,0,9.11,0s9.11,4.04,9.11,9c0,2.12-.77,4.18-2.13,5.79l7.61,7.5c.39,.39,.4,1.02,0,1.41-.2,.2-.45,.3-.71,.3ZM9.11,2c-3.92,0-7.11,3.14-7.11,7s3.19,7,7.11,7c1.83,0,3.57-.68,4.89-1.92,0,0,0,0,0,0,1.43-1.33,2.21-3.14,2.21-5.08,0-3.86-3.19-7-7.11-7Z"></path> </svg> </label> <input class="" type="search" placeholder="Search pages or people" aria-label="Search pages or people" name="term" id="QUsearch" /> </div> </form> </section> </section> </section> </section></div> </div> </div> </div> </div> </div> <!--Site Title--> <div class="branding"> <div class="container"> <div class="region region-branding"> <div id="block-sitetitle" class="block block-block-content block-block-contentc3ecb934-0770-436d-ad29-6e876211d7a0 no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><a class="site-title" href="/">Biomedical and Molecular Sciences</a> </div> </div> </div> </div> <!--Mobile Menu Toggle Button --> <div id="menu-bar" class="menu-bar hidden-lg hidden-md"> <span class="one"></span> <span class="two"></span> <span class="three"></span> </div> </div> </div> <!--Main Menu--> <div class="area-main-menu"> <div class="area-inner container"> <div class="gva-offcanvas-mobile"> <div class="close-offcanvas hidden"> <i class="fa fa-times"></i> </div> <div class="region region-main-menu"> <nav role="navigation" aria-labelledby="block-gavias-financial-mainnavigation-menu" id="block-gavias-financial-mainnavigation" class="header-height block block-menu navigation menu--main"> <h2 class="visually-hidden block-title" id="block-gavias-financial-mainnavigation-menu"><span>Main navigation</span></h2> <div class="block-content"> <div class="gva-navigation"> <ul class="clearfix gva_menu gva_menu_main"> <li class="menu-item menu-item--expanded "> <a href="/about"> Administration <span class="icaret nav-plus fa fa-angle-down"></span> </a> <ul class="menu sub-menu"> <li class="menu-item"> <a href="/about"> Welcome to DBMS </a> </li> <li class="menu-item"> <a href="/about/human-body-donor-program"> Human Body Donor Program </a> </li> <li class="menu-item menu-item--collapsed"> <a href="/about/services"> Services </a> </li> <li class="menu-item"> <a href="/administration/faculty"> For Faculty </a> </li> <li class="menu-item"> <a href="/contact-us"> Contact us </a> </li> <li class="menu-item"> <a href="https://healthsci.queensu.ca/opdes/cpd/educational-programs-opportunities/human-anatomy-dissection-experience"> Human Anatomy Dissection Experience </a> </li> <li class="menu-item"> <a href="https://www.seamo.ca/physician-wellness"> Physician Wellness </a> </li> </ul> </li> <li class="menu-item menu-item--expanded "> <a href="/undergraduate"> Undergraduate <span class="icaret nav-plus fa fa-angle-down"></span> </a> <ul class="menu sub-menu"> <li class="menu-item"> <a href="/undergraduate/courses"> Courses </a> </li> <li class="menu-item"> <a href="https://healthsci.queensu.ca/liscbchm/"> Life Sciences & Biochemistry </a> </li> <li class="menu-item"> <a href="https://bhsc.queensu.ca/"> Bachelor of Health Sciences </a> </li> <li class="menu-item"> <a href="/undergraduate/student-lab-schedule"> Student Lab Schedule </a> </li> <li class="menu-item"> <a href="/undergraduate/jd-hatcher-award"> J.D. Hatcher Award </a> </li> <li class="menu-item"> <a href="/undergraduate/tutors"> Tutors </a> </li> <li class="menu-item"> <a href="/undergraduate/nserc-usra"> NSERC USRA </a> </li> <li class="menu-item"> <a href="/undergraduate/ussrf"> USSRF </a> </li> </ul> </li> <li class="menu-item menu-item--expanded "> <a href="/graduate"> Graduate <span class="icaret nav-plus fa fa-angle-down"></span> </a> <ul class="menu sub-menu"> <li class="menu-item menu-item--collapsed"> <a href="/graduate/programs"> Programs </a> </li> <li class="menu-item menu-item--collapsed"> <a href="/graduate/prospective-students"> Prospective Students </a> </li> <li class="menu-item"> <a href="/graduate/current-students"> Current Students </a> </li> <li class="menu-item"> <a href="/graduate/graduate-student-handbook"> Graduate Student Handbook </a> </li> <li class="menu-item menu-item--collapsed"> <a href="/graduate/teaching-and-academic-assistant-positions"> Teaching and Academic Assistant Positions </a> </li> <li class="menu-item"> <a href="/graduate/graduate-courses"> Courses </a> </li> <li class="menu-item"> <a href="/graduate/contact-us"> Contact us </a> </li> </ul> </li> <li class="menu-item "> <a href="/faculty"> Faculty </a> </li> <li class="menu-item menu-item--expanded menu-item--active-trail "> <a href="/research-groups"> Research <span class="icaret nav-plus fa fa-angle-down"></span> </a> <ul class="menu sub-menu"> <li class="menu-item menu-item--collapsed menu-item--active-trail"> <a href="/research/core-research-facilities"> Core Research Facilities </a> </li> <li class="menu-item"> <a href="/research/research-day"> Research Day </a> </li> <li class="menu-item"> <a href="http://qcri.queensu.ca/"> Cancer Research Institute </a> </li> <li class="menu-item"> <a href="https://deptmed.queensu.ca/research/teams/qcpu"> Queen's Cardiopulmonary Research Unit </a> </li> <li class="menu-item"> <a href="http://neuroscience.queensu.ca/"> Centre for Neuroscience Studies </a> </li> <li class="menu-item"> <a href="https://deptmed.queensu.ca/research/teams/gidru"> Gastrointestinal Diseases Research Unit </a> </li> <li class="menu-item"> <a href="/research-groups/reproductive-and-developmental"> Group for Research in Reproduction and Development </a> </li> <li class="menu-item"> <a href="/research-groups/infection-immunity-and-inflammation-research"> Infection, Immunity and Inflammation Research </a> </li> </ul> </li> <li class="menu-item"> <a href="/seminar-series"> Seminar Series </a> </li> <li class="menu-item "> <a href="/job-opportunities"> Job Opportunities </a> </li> <li class="menu-item "> <a href="https://dbms.queensu.ca/contact-us"> Contact us </a> </li> </ul> </div> </div> </nav> </div> </div> </div> </div> </div> </div> </header> <div class="breadcrumbs"> <div class="region region-breadcrumbs"> <div class="breadcrumb-content-inner"> <div class="gva-breadcrumb-content"> <div id="block-gavias-financial-breadcrumbs" class="text-dark block gva-block-breadcrumb hidden-xs block-system block-system-breadcrumb-block no-title"> <div class="container"> <div class="breadcrumb-content-main"> <h2 class="page-title hide">Fast Purification Liquid Chromatography (FPLC) </h2> <div class=""> <div class="content block-content"> <div class="breadcrumb-links"> <div class="content-inner"> <nav class="breadcrumb " role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> <span class=""> / </span> </li> <li> <a href="/node"></a> <span class=""> / </span> </li> <li> </li> <li> Fast Purification Liquid Chromatography (FPLC) </li> <li></li> </ol> </nav> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div role="main" class="main main-page"> <div class="clearfix"></div> <div class="clearfix"></div> <div class="clearfix"></div> <div id="content" class="content content-full"> <div class="container-full"> <div class="content-main-inner"> <div id="page-main-content" class="main-content"> <div class="main-content-inner"> <div class="content-main"> <div class="region region-content"> <div id="block-gavias-financial-content" class="block block-system block-system-main-block no-title"> <div class="content block-content"> <!-- Start Display article for detail page --> <article data-history-node-id="1075" typeof="schema:WebPage" class="node node--type-page node--view-mode-full"> <header> <div class="container"> <div class="widget gsc-heading align-left style-1 small text-dark "> <h1 class="title"><span><span property="schema:name" class="field field--name-title field--type-string field--label-hidden">Fast Purification Liquid Chromatography (FPLC)</span> </span></h1> <div class="line"><span class="one"></span><span class="second"></span><span class="three"></span></div> </div> </div> </header> <div class="node__content clearfix"> </div> <div class="gavias-content-blockbuilder"><div class="gavias-blockbuilder-content"> <div class="gbb-row-wrapper"> <div class=" gbb-row bg-size-cover" style="" > <div class="bb-inner default"> <div class="bb-container container"> <div class="row"> <div class="row-wrapper clearfix"> <div class="gsc-column col-lg-12 col-md-12 col-sm-12 col-xs-12"> <div class="column-inner bg-size-cover " > <div class="column-content-inner"> <div class="column-content "><h2>AKTA Explorer</h2></div> <div class="widget gsc-image " > <div class="widget-content"> <img src="https://dbms.queensu.ca/sites/dbms/files/gbb-uploads/AKTA Explorer_Instrument-sg37zt.png" alt="" /> </div> </div> <div class="gsc-tabs "> <div class="tabs_wrapper tabs_vertical"> <ul class="nav nav-tabs"> <li class="active"><a data-toggle="tab" href="#uid-7kria-1"> Description </a></li> <li ><a data-toggle="tab" href="#uid-7kria-2"> Features </a></li> <li ><a data-toggle="tab" href="#uid-7kria-3"> Booking & Fees </a></li> </ul> <div class="tab-content"> <div id="uid-7kria-1" class="tab-pane fade in active"><p>The AKTA Explorer is designed to be compatible with a variety of chromatography columns and protein purification strategies. Available columns include Superdex 75 and 200 columns to separate small (analytical) or large (preparative) quantities of protein. Ion exchange columns including Q and SP Sepharose are available to purify proteins based on their charges. Columns containing affinity capture resins (e.g. Ni-NTA) can also be used if they have compatible fittings.</p></div> <div id="uid-7kria-2" class="tab-pane fade in "><p>The AKTA Explorer is controlled by an external computer using the Unicorn 5.31 operating system. The FPLC can detect protein content using UV Absorbance, and can also measure pH and conductivity for gradient-based purifications. Fractions of interest can be collected via the Frac-950. Maximum flow rate = 100 ml/min. Maximum pressure = 2 MPa (depending on the column used).</p></div> <div id="uid-7kria-3" class="tab-pane fade in "><p><strong>Fees</strong></p> <table style="width: 525px;"> <tbody> <tr> <td style="width: 259.609px;">Service</td> <td style="width: 269.391px;">Fee</td> </tr> <tr> <td style="width: 259.609px;">Instrument</td> <td style="width: 269.391px;">Free</td> </tr> <tr> <td style="width: 259.609px;">Training</td> <td style="width: 269.391px;">$125/session</td> </tr> </tbody> </table> <p><strong>Contact and Booking </strong></p> <p>Xiaohu Yan (<a href="mailto:yanx@queensu.ca">yanx@queensu.ca</a>; ext. 78265) and Zhilin Chen (<a href="mailto:zc@queensu.ca">zc@queensu.ca</a>; ext. 78238)</p> <p><strong>Location </strong></p> <p>Botterell Hall, Room 667</p> <p> </p></div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div></div></div> </article> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <footer id="footer" class="footer"> <div class="footer-inner"> <div class="footer-center"> <div class="container"> <div class="row"> <div class="footer-first col-lg-3 col-md-3 col-sm-12 col-xs-12 column"> <div class="region region-footer-first"> <div id="block-queensfooterlogo-2" class="block block-block-content block-block-contentf665d6b6-d3bf-4022-bc0c-d81efa4c3c55 no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="qu_footer_branding"></section></div> </div> </div> </div> </div> <div class="footer-second col-lg-9 col-md-9 col-sm-12 col-xs-12 column"> <div class="region region-footer-second"> <div id="block-linkfooter" class="hidden-xs hidden-sm block block-block-content block-block-contente03afdfc-b255-481b-9605-5a0e153ccacf no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="qhs_schools"></section></div> </div> </div> </div> </div> <div class="footer-third col-lg-12 col-md-12 col-sm-12 col-xs-12 column"> <div class="region region-footer-third"> <div id="block-contactinfomschq" class="block block-block-content block-block-contentfc5b443f-0626-414c-bca6-320b99b79ec2 no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="site_contact_social"> <section> <p class="title">Contact Us</p> <ul class="contact"> <li> <a href="/contact-us"><i class="fas fa-home"></i>Department of Biomedical and Molecular Sciences</a> </li> <li><i class="fas fa-phone"></i>(613)-533-2600</li> <li> <a href="mailto:dbms@queensu.ca"><i class="fas fa-envelope"></i>dbms@queensu.ca</a> </li> </ul> </section> <section> <p class="title">Follow Us</p> <ul class="social"> <li> <a href="https://twitter.com/dbmstweets" target="_blank"><i class="fa fa-twitter"></i></a> </li> </ul> </section> </section></div> </div> </div> </div> </div> <div class="footer-four col-lg-12 col-md-12 col-sm-12 col-xs-12 column"> <div class="region region-footer-four"> <div id="block-partnerhospitals" class="block block-block-content block-block-contentcff6a208-af38-48d2-a67a-b0d2c3b6d6ae no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="qhs_hospitals"></section></div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="copyright"> <div class="container"> <div class="copyright-inner"> <div class="region region-copyright"> <div id="block-gavias-financial-copyright" class="block block-block-content block-block-content6a74724d-d485-4216-a560-962e52758fde no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="qhs_copyright"></section></div> </div> </div> </div> </div> </div> </div> </footer> </div> <script src="/sites/dbms/files/js/js_R7ozUuXOSWqf1_7irlZ-puSWIjD4C0bt_k5JZ96HirE.js?scope=footer&delta=0&language=en&theme=gavias_financial&include=eJx1kGFuwzAIhS_k2keKiENcFmxXQKp5p5-lNktXq__ge_CeQBEkXifYrcaab4yGwa6Y0WcqlIF9VHWxCoZF9lvvX0edNjXMYQZFFxlU2786oyokfBqUKt2QftAluBPotFKBEgk4JK4z8EWtMZU06l96WWs1lFGqdxShpYeMrg9wTuC3df_tOOXZOjUwUqOoh3IS138hCUtsEzCKaXgHR65yzxCGhhJG5PtD0NSvUothWY6tmWvc5p14Odde2ae94co_4HWj4mfe8Rcntrw3"></script> <script src="https://www.queensu.ca/resources/emergencyalerts/js/axios.min.js" defer=""></script> <script src="https://www.queensu.ca/resources/emergencyalerts/js/react.production.min.js" defer=""></script> <script src="https://www.queensu.ca/resources/emergencyalerts/js/react-dom.production.min.js" defer=""></script> <script src="https://www.queensu.ca/resources/emergencyalerts/js/emergencyalerts.js" defer=""></script> <script src="/sites/dbms/files/js/js_9lEcci7E7C32BEDTlugbto51kQzjDyrk-YNmfl85w1Q.js?scope=footer&delta=5&language=en&theme=gavias_financial&include=eJx1kGFuwzAIhS_k2keKiENcFmxXQKp5p5-lNktXq__ge_CeQBEkXifYrcaab4yGwa6Y0WcqlIF9VHWxCoZF9lvvX0edNjXMYQZFFxlU2786oyokfBqUKt2QftAluBPotFKBEgk4JK4z8EWtMZU06l96WWs1lFGqdxShpYeMrg9wTuC3df_tOOXZOjUwUqOoh3IS138hCUtsEzCKaXgHR65yzxCGhhJG5PtD0NSvUothWY6tmWvc5p14Odde2ae94co_4HWj4mfe8Rcntrw3"></script> </body> </html>