Its high speed sorting capabilities and flexible configuration allow for target cells to be purified into a range of collection tubes, and also 6-384 well plates, with the added capability of single cell deposition into wells for clone assays and also to sort defined numbers and populations onto slides for subsequent microscopy analysis.
The facility Aria is currently fitted with 488nm and 633nm lasers combined with a detector array of 2 scatter channels and up to 8 simultaneous colours from 525-775nm.
488 laser: 530nm (fitc), 585nm(pe), 616nm(pe tex red), 695nm(percp), 780nm(pe-cy7)
633 laser: 660nm (apc), 730 (alexa700), 780(apc-cy 7)
Fees
Service
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Cost
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Instrument use (with technical assistance)
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$90/hr
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Contact and Booking
Zhilin Chen (zc@queensu.ca; ext. 78238) and Jeffrey Mewburn (mewburnj@queensu.ca; ext. 75389)
Flow sorting book AM on TUES, WED or THURS, Please book the appointments at least 1 week in advance.
Location
Botterell Hall, Rm 314/315, housed in a BH level 2 compliant hood.