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hidden-xs block-system block-system-breadcrumb-block no-title"> <div class="container"> <div class="breadcrumb-content-main"> <h2 class="page-title hide">CytoFLEX </h2> <div class=""> <div class="content block-content"> <div class="breadcrumb-links"> <div class="content-inner"> <nav class="breadcrumb " role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> <span class=""> / </span> </li> <li> <a href="/research/core-research-facilities">Core Research Facilities</a> <span class=""> / </span> </li> <li> <a href="/research/core-research-facilities/flow-cytometry-core-facility">Flow Cytometry Core Facility</a> <span class=""> / </span> </li> <li> </li> <li> CytoFLEX </li> <li></li> </ol> </nav> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div role="main" class="main main-page"> <div class="clearfix"></div> <div class="clearfix"></div> <div 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</div> <div class="gavias-content-blockbuilder"><div class="gavias-blockbuilder-content"> <div class="gbb-row-wrapper"> <div class=" gbb-row bg-size-cover" style="" > <div class="bb-inner default"> <div class="bb-container container"> <div class="row"> <div class="row-wrapper clearfix"> <div class="gsc-column col-lg-12 col-md-12 col-sm-12 col-xs-12"> <div class="column-inner bg-size-cover " > <div class="column-content-inner"> <div class="widget gsc-image " > <div class="widget-content"> <img src="https://dbms.queensu.ca/sites/dbms/files/gbb-uploads/CytoFLEX-s4pd1z.png" alt="" /> </div> </div> <div class="gsc-tabs "> <div class="tabs_wrapper tabs_vertical"> <ul class="nav nav-tabs"> <li class="active"><a data-toggle="tab" href="#uid-d6zo0-1"> Description </a></li> <li ><a data-toggle="tab" href="#uid-d6zo0-2"> Features </a></li> <li ><a data-toggle="tab" href="#uid-d6zo0-3"> Booking & Fees </a></li> </ul> <div class="tab-content"> <div id="uid-d6zo0-1" class="tab-pane fade in active"><p>CytoFLEX flow cytometers and cell sorters provide rapid, multi-parametric analysis of cell populations in solution, which can be analyzed and/or purified based on their fluorescent or light scattering characteristics. This powerful tool enables the user to perform immunophenotyping panels, cell cycle analysis, and measure apoptosis. These functions have applications in immunology, molecular biology, bacteriology, virology, cancer biology and infectious disease monitoring.</p></div> <div id="uid-d6zo0-2" class="tab-pane fade in "><p>We have two instruments.</p> <p><strong> </strong></p> <p>CytoFLEX 1: Beckman Cytoflex S is a 4 laser (Violet-Blue-Yellow Green-Red) flow cytometer allowing analysis of samples in a multiwell round bottom plate (up to 13 fluorophores).</p> <p> </p> <p>CytoFLEX 2: Beckman Cytoflex is a 3 laser (Violet-Blue-Red) flow cytometer allowing analysis of samples in a 12 x 75 mm tube format (up to 10 fluorophores).</p></div> <div id="uid-d6zo0-3" class="tab-pane fade in "><p><strong>Fees</strong></p> <table style="width: 51.6742%;"> <tbody> <tr> <td style="width: 32%;"> <p><strong>Service</strong></p> </td> <td style="width: 17.6742%;"> <p><strong>Cost</strong></p> </td> </tr> <tr> <td style="width: 32%;"> <p>Instrument use (independent use)</p> </td> <td style="width: 17.6742%;"> <p>$30/hr</p> </td> </tr> <tr> <td style="width: 32%;"> <p>Training</p> </td> <td style="width: 17.6742%;"> <p>$50/session</p> </td> </tr> </tbody> </table> <p><strong>Contact and Booking </strong></p> <p>Zhilin Chen (<a href="mailto:zc@queensu.ca">zc@queensu.ca</a>; ext. 78238) and Jeffrey Mewburn (<a href="mailto:mewburnj@queensu.ca">mewburnj@queensu.ca</a>; ext. 75389)</p> <p><u>Notes on equipment use</u>:</p> <ul> <li>New users will receive training from Zhilin or Xiaohu prior to booking & running experiments</li> <li>Filter locations are programmed into the software and should not be changed; all users with samples in tubes will need to consult staff to ensure system is switched back to plate mode</li> <li>Staff will run calibration beads on days the instrument is booked</li> <li>Evening & weekend calendar bookings will be restricted to persons with documented training & require their own calibration beads; if working evenings or weekends, a key for Rm 569 can be requested but will be revoked if not following proper cleaning and shutdown procedures</li> <li>Mouse cell samples can be run without fixation, but most human cell (or microbial) samples will need to be fixed prior to entering Rm 569; if you are unsure of the biosafety level of your samples please consult EHS</li> <li>Data analysis should be done remotely. Priority for the use of the computer will be dedicated to the operation of the cytoflex and data acquisition</li> <li>All users need to perform the proper shutdown procedures as posted. Please turn the lights off and ensure the door is locked when you are done</li> </ul> <p><strong>Location</strong></p> <p>Botterell Hall Rm 569</p> <p> </p></div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div></div></div> </article> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <footer id="footer" class="footer"> <div class="footer-inner"> <div class="footer-center"> <div class="container"> <div class="row"> <div class="footer-first col-lg-3 col-md-3 col-sm-12 col-xs-12 column"> <div class="region region-footer-first"> <div id="block-queensfooterlogo-2" class="block block-block-content block-block-contentf665d6b6-d3bf-4022-bc0c-d81efa4c3c55 no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="qu_footer_branding"></section></div> </div> </div> </div> </div> <div class="footer-second col-lg-9 col-md-9 col-sm-12 col-xs-12 column"> <div class="region region-footer-second"> <div id="block-linkfooter" class="hidden-xs hidden-sm block block-block-content block-block-contente03afdfc-b255-481b-9605-5a0e153ccacf no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="qhs_schools"></section></div> </div> </div> </div> </div> <div class="footer-third col-lg-12 col-md-12 col-sm-12 col-xs-12 column"> <div class="region region-footer-third"> <div id="block-contactinfomschq" class="block block-block-content block-block-contentfc5b443f-0626-414c-bca6-320b99b79ec2 no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="site_contact_social"> <section> <p class="title">Contact Us</p> <ul class="contact"> <li> <a href="/contact-us"><i class="fas fa-home"></i>Department of Biomedical and Molecular Sciences</a> </li> <li><i class="fas fa-phone"></i>(613)-533-2600</li> <li> <a href="mailto:dbms@queensu.ca"><i class="fas fa-envelope"></i>dbms@queensu.ca</a> </li> </ul> </section> <section> <p class="title">Follow Us</p> <ul class="social"> <li> <a href="https://twitter.com/dbmstweets" target="_blank"><i class="fa fa-twitter"></i></a> </li> </ul> </section> </section></div> </div> </div> </div> </div> <div class="footer-four col-lg-12 col-md-12 col-sm-12 col-xs-12 column"> <div class="region region-footer-four"> <div id="block-partnerhospitals" class="block block-block-content block-block-contentcff6a208-af38-48d2-a67a-b0d2c3b6d6ae no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="qhs_hospitals"></section></div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="copyright"> <div class="container"> <div class="copyright-inner"> <div class="region region-copyright"> <div id="block-gavias-financial-copyright" class="block block-block-content block-block-content6a74724d-d485-4216-a560-962e52758fde no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="qhs_copyright"></section></div> </div> </div> </div> </div> </div> </div> </footer> </div> <script src="/sites/dbms/files/js/js_R7ozUuXOSWqf1_7irlZ-puSWIjD4C0bt_k5JZ96HirE.js?scope=footer&delta=0&language=en&theme=gavias_financial&include=eJx1kGFuwzAIhS_k2keKiENcFmxXQKp5p5-lNktXq__ge_CeQBEkXifYrcaab4yGwa6Y0WcqlIF9VHWxCoZF9lvvX0edNjXMYQZFFxlU2786oyokfBqUKt2QftAluBPotFKBEgk4JK4z8EWtMZU06l96WWs1lFGqdxShpYeMrg9wTuC3df_tOOXZOjUwUqOoh3IS138hCUtsEzCKaXgHR65yzxCGhhJG5PtD0NSvUothWY6tmWvc5p14Odde2ae94co_4HWj4mfe8Rcntrw3"></script> <script src="https://www.queensu.ca/resources/emergencyalerts/js/axios.min.js" defer=""></script> <script src="https://www.queensu.ca/resources/emergencyalerts/js/react.production.min.js" defer=""></script> <script src="https://www.queensu.ca/resources/emergencyalerts/js/react-dom.production.min.js" defer=""></script> <script src="https://www.queensu.ca/resources/emergencyalerts/js/emergencyalerts.js" defer=""></script> <script src="/sites/dbms/files/js/js_9lEcci7E7C32BEDTlugbto51kQzjDyrk-YNmfl85w1Q.js?scope=footer&delta=5&language=en&theme=gavias_financial&include=eJx1kGFuwzAIhS_k2keKiENcFmxXQKp5p5-lNktXq__ge_CeQBEkXifYrcaab4yGwa6Y0WcqlIF9VHWxCoZF9lvvX0edNjXMYQZFFxlU2786oyokfBqUKt2QftAluBPotFKBEgk4JK4z8EWtMZU06l96WWs1lFGqdxShpYeMrg9wTuC3df_tOOXZOjUwUqOoh3IS138hCUtsEzCKaXgHR65yzxCGhhJG5PtD0NSvUothWY6tmWvc5p14Odde2ae94co_4HWj4mfe8Rcntrw3"></script> </body> </html>