SD TIRF - Spinning Disc Confocal w/ Total Internal Reflectance of Fluorescence module | Biomedical and Molecular Sciences | School of Medicine

The Quorum spinning disc confocal is a filter and camera driven confocal microscope with extremely high acquisition rates and enhanced 3d imaging functionality. The SD technology lends itself to 3d and live cell/tissue work particularly and the scope is consequently equipped with a gas, temperature and humidity controlled environment chamber for extended in vivo time lapse experiments.

Also equipped is a fully motorised stage which allows for the repeated acquisition of mutiple, user defined fields of view which can be extended to the analysis of treated vs control conditions using a double slide holder. This lends itself to the generation of multiple 4D data sets within a single experiment,

This is a particularly powerful instrument for such large scale experiments and the speed of acquisition even allows for the scopes use as a high resolution fluorescent intravital setup, for measuring the behaviour of vascular phenomena in situ and in real time.

The SD is run through Metamorph software, which has inbuilt modules for the easy assessment of such large data sets as well as full image analysis and measurement capability, efficient 3d reconstruction, and deconvolution modules and a user friendly interface. .

The scope is equipped with 405, 440, 491, 561 and 640nm diode laser excitation lines, and 460 470 520 590 620 and 690nm peak detection filters, acquired with a Hamamatsu Orca high resolution camera.This system is also equipped with a TIRF module (see applications)

Fees:

Service	Cost
Spin Disk Microscope (Assited)	$50/hr
Spin Disk Microscope (Unassisted)	$30/hr
SD TIRF (Assited)	$50/hr
SD TIRF (Unassisted)	$30/hr
Training	$50/session

Contact and Booking

Nichole Peterson (maletten@queensu.ca; ext. 32853) and Jeffrey Mewburn (mewburnj@queensu.ca; ext. 75389)

Location

Botterell Hall, Rm 322