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hidden-xs block-system block-system-breadcrumb-block no-title"> <div class="container"> <div class="breadcrumb-content-main"> <h2 class="page-title hide">Droplet Digital PCR (ddPCR) Technology </h2> <div class=""> <div class="content block-content"> <div class="breadcrumb-links"> <div class="content-inner"> <nav class="breadcrumb " role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> <span class=""> / </span> </li> <li> <a href="/research/core-research-facilities">Core Research Facilities</a> <span class=""> / </span> </li> <li> <a href="/research/core-research-facilities/molecular-biology-core-facility">Molecular Biology Core Facility</a> <span class=""> / </span> </li> <li> </li> <li> Droplet Digital PCR (ddPCR) Technology </li> <li></li> </ol> </nav> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div role="main" class="main main-page"> <div class="clearfix"></div> <div class="clearfix"></div> <div class="clearfix"></div> <div id="content" class="content content-full"> <div class="container-full"> <div class="content-main-inner"> <div id="page-main-content" class="main-content"> <div class="main-content-inner"> <div class="content-main"> <div class="region region-content"> <div id="block-gavias-financial-content" class="block block-system block-system-main-block no-title"> <div class="content block-content"> <!-- Start Display article for detail page --> <article data-history-node-id="1087" typeof="schema:WebPage" class="node node--type-page node--view-mode-full"> <header> <div class="container"> <div class="widget gsc-heading align-left style-1 small text-dark "> <h1 class="title"><span><span property="schema:name" class="field field--name-title field--type-string field--label-hidden">Droplet Digital PCR (ddPCR) Technology</span> </span></h1> <div class="line"><span class="one"></span><span class="second"></span><span class="three"></span></div> </div> </div> </header> <div class="node__content clearfix"> </div> <div class="gavias-content-blockbuilder"><div class="gavias-blockbuilder-content"> <div class="gbb-row-wrapper"> <div class=" gbb-row bg-size-cover" style="" > <div class="bb-inner default"> <div class="bb-container container"> <div class="row"> <div class="row-wrapper clearfix"> <div class="gsc-column col-lg-12 col-md-12 col-sm-12 col-xs-12"> <div class="column-inner bg-size-cover " > <div class="column-content-inner"> <div class="gsc-our-gallery "> <div class="owl-carousel init-carousel-owl owl-loaded owl-drag" data-items="1" data-items_lg="1" data-items_md="1" data-items_sm="2" data-items_xs="1" data-loop="1" data-speed="500" data-auto_play="1" data-auto_play_speed="2000" data-auto_play_timeout="5000" data-auto_play_hover="1" data-navigation="1" data-rewind_nav="0" data-pagination="1" data-mouse_drag="1" data-touch_drag="1"> <div class="item"><div class="content-inner"> <div class="image"><img src="https://dbms.queensu.ca/sites/dbms/files/gbb-uploads/ddPCR-1-lszy3t-wimzsy.png" alt=" Droplet Digital PCR (ddPCR) Technology "/></div> </div></div> <div class="item"><div class="content-inner"> <div class="image"><img src="https://dbms.queensu.ca/sites/dbms/files/gbb-uploads/ddPCR-2-u4gyzv-fnqhxw.png" alt=" Droplet Digital PCR (ddPCR) Technology "/></div> </div></div> </div> </div> <div class="gsc-tabs "> <div class="tabs_wrapper tabs_vertical"> <ul class="nav nav-tabs"> <li class="active"><a data-toggle="tab" href="#uid-toirl-1"> Description </a></li> <li ><a data-toggle="tab" href="#uid-toirl-2"> Features </a></li> <li ><a data-toggle="tab" href="#uid-toirl-3"> Booking & Fees </a></li> </ul> <div class="tab-content"> <div id="uid-toirl-1" class="tab-pane fade in active"><p>Droplet Digital PCR (ddPCR) is a method for performing digital PCR that is based on water-oil emulsion droplet technology. A sample is fractionated into 20,000 droplets, and PCR amplification of the template molecules occurs in each individual droplet. Following PCR, each droplet is analyzed or read to determine the fraction of PCR-positive droplets in the original sample. These data are then analyzed to determine the target DNA template concentration in the original sample.</p></div> <div id="uid-toirl-2" class="tab-pane fade in "><p>Bio-Rad’s <a href="https://www.bio-rad.com/en-us/product/qx200-droplet-digital-pcr-system">QX200 Droplet Digital PCR (ddPCR) System</a> can be used to:</p> <ul> <li>Detect rare DNA target copies with unmatched sensitivity</li> <li>Determine copy number variation with unrivaled accuracy</li> <li>Measure gene expression levels with exquisite precision</li> </ul></div> <div id="uid-toirl-3" class="tab-pane fade in "><p><strong>Fees</strong></p> <table style="height: 82px; width: 341px;"> <tbody> <tr> <td style="width: 144px;"><strong>Service</strong></td> <td style="width: 181px;"><strong>Cost</strong></td> </tr> <tr> <td style="width: 144px;">Instrument </td> <td style="width: 181px;">$10/8 samples</td> </tr> <tr> <td style="width: 144px;">Training </td> <td style="width: 181px;">$50/session</td> </tr> </tbody> </table> <p><strong>Contact and Booking</strong></p> <p>Zhilin Chen (<a href="mailto:zc@queensu.ca">zc@queensu.ca</a>; ext. 78238) and Christie Gilmour (<a class="mailto" href="mailto:gilmourc@queensu.ca" data-extlink="">gilmourc@queensu.ca</a>; ext. 36487)</p> <p><strong>Location</strong></p> <p>Botterell Hall Room 549</p> <p> </p> <p> </p></div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div></div></div> </article> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <footer id="footer" class="footer"> <div class="footer-inner"> <div class="footer-center"> <div class="container"> <div class="row"> <div class="footer-first col-lg-3 col-md-3 col-sm-12 col-xs-12 column"> <div class="region region-footer-first"> <div id="block-queensfooterlogo-2" class="block block-block-content block-block-contentf665d6b6-d3bf-4022-bc0c-d81efa4c3c55 no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="qu_footer_branding"></section></div> </div> </div> </div> </div> <div class="footer-second col-lg-9 col-md-9 col-sm-12 col-xs-12 column"> <div class="region region-footer-second"> <div id="block-linkfooter" class="hidden-xs hidden-sm block block-block-content block-block-contente03afdfc-b255-481b-9605-5a0e153ccacf no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="qhs_schools"></section></div> </div> </div> </div> </div> <div class="footer-third col-lg-12 col-md-12 col-sm-12 col-xs-12 column"> <div class="region region-footer-third"> <div id="block-contactinfomschq" class="block block-block-content block-block-contentfc5b443f-0626-414c-bca6-320b99b79ec2 no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="site_contact_social"> <section> <p class="title">Contact Us</p> <ul class="contact"> <li> <a href="/contact-us"><i class="fas fa-home"></i>Department of Biomedical and Molecular Sciences</a> </li> <li><i class="fas fa-phone"></i>(613)-533-2600</li> <li> <a href="mailto:dbms@queensu.ca"><i class="fas fa-envelope"></i>dbms@queensu.ca</a> </li> </ul> </section> <section> <p class="title">Follow Us</p> <ul class="social"> <li> <a href="https://twitter.com/dbmstweets" target="_blank"><i class="fa fa-twitter"></i></a> </li> </ul> </section> </section></div> </div> </div> </div> </div> <div class="footer-four col-lg-12 col-md-12 col-sm-12 col-xs-12 column"> <div class="region region-footer-four"> <div id="block-partnerhospitals" class="block block-block-content block-block-contentcff6a208-af38-48d2-a67a-b0d2c3b6d6ae no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="qhs_hospitals"></section></div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="copyright"> <div class="container"> <div class="copyright-inner"> <div class="region region-copyright"> <div id="block-gavias-financial-copyright" class="block block-block-content block-block-content6a74724d-d485-4216-a560-962e52758fde no-title"> <div class="content block-content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><section id="qhs_copyright"></section></div> </div> </div> </div> </div> </div> </div> </footer> </div> <script src="/sites/dbms/files/js/js_R7ozUuXOSWqf1_7irlZ-puSWIjD4C0bt_k5JZ96HirE.js?scope=footer&delta=0&language=en&theme=gavias_financial&include=eJx1kGFuwzAIhS_k2keKiENcFmxXQKp5p5-lNktXq__ge_CeQBEkXifYrcaab4yGwa6Y0WcqlIF9VHWxCoZF9lvvX0edNjXMYQZFFxlU2786oyokfBqUKt2QftAluBPotFKBEgk4JK4z8EWtMZU06l96WWs1lFGqdxShpYeMrg9wTuC3df_tOOXZOjUwUqOoh3IS138hCUtsEzCKaXgHR65yzxCGhhJG5PtD0NSvUothWY6tmWvc5p14Odde2ae94co_4HWj4mfe8Rcntrw3"></script> <script src="https://www.queensu.ca/resources/emergencyalerts/js/axios.min.js" defer=""></script> <script src="https://www.queensu.ca/resources/emergencyalerts/js/react.production.min.js" defer=""></script> <script src="https://www.queensu.ca/resources/emergencyalerts/js/react-dom.production.min.js" defer=""></script> <script src="https://www.queensu.ca/resources/emergencyalerts/js/emergencyalerts.js" defer=""></script> <script src="/sites/dbms/files/js/js_9lEcci7E7C32BEDTlugbto51kQzjDyrk-YNmfl85w1Q.js?scope=footer&delta=5&language=en&theme=gavias_financial&include=eJx1kGFuwzAIhS_k2keKiENcFmxXQKp5p5-lNktXq__ge_CeQBEkXifYrcaab4yGwa6Y0WcqlIF9VHWxCoZF9lvvX0edNjXMYQZFFxlU2786oyokfBqUKt2QftAluBPotFKBEgk4JK4z8EWtMZU06l96WWs1lFGqdxShpYeMrg9wTuC3df_tOOXZOjUwUqOoh3IS138hCUtsEzCKaXgHR65yzxCGhhJG5PtD0NSvUothWY6tmWvc5p14Odde2ae94co_4HWj4mfe8Rcntrw3"></script> </body> </html>