Droplet Digital PCR (ddPCR) is a method for performing digital PCR that is based on water-oil emulsion droplet technology. A sample is fractionated into 20,000 droplets, and PCR amplification of the template molecules occurs in each individual droplet. Following PCR, each droplet is analyzed or read to determine the fraction of PCR-positive droplets in the original sample. These data are then analyzed to determine the target DNA template concentration in the original sample.
Bio-Rad’s QX200 Droplet Digital PCR (ddPCR) System can be used to:
- Detect rare DNA target copies with unmatched sensitivity
- Determine copy number variation with unrivaled accuracy
- Measure gene expression levels with exquisite precision
Fees
Service |
Cost |
Instrument |
$10/8 samples |
Training |
$50/session |
Contact and Booking
Zhilin Chen (zc@queensu.ca; ext. 78238) and Christie Gilmour (gilmourc@queensu.ca; ext. 36487)
Location
Botterell Hall Room 549