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BCHM 315 - Proteins and Enzymes

 Fall 2022
 Year 3
 3-4 hours/week
 3 units
On-Campus Course

Principles of protein biochemistry, enzymology, and protein engineering.

Prerequisites: Level 3 or above and BCHM 218/3.0 and [(CHEM 222/3.0 and CHEM 223/3.0) or CHEM 282/3.0].

NOTE Students lacking the prerequisites CHEM 222/3.0 or CHEM 282/3.0 may take these courses as a corequisite with permission of the Department.

BCHM 102/3.0; BCHM 310/9.0

After completing this course, students will be able to:

  1. Describe the charge state of an amino acid and explain how that relates to its behaviour.
  2. Describe how the charge state of a protein depends on its pI and the pH of the environment and explain how that relates to its behaviour.
  3. Describe how proteins can be purified using a variety of methods.
  4. Describe the different levels of protein structure (primary, secondary, etc.) and explain the important forces that stabilize them.
  5. Describe the expected results of experiments that could be used to study protein structure and function.
  6. Design experiments that could be used to study protein structure and function.
  7. Explain how the sequences of a variety of related proteins can be used to understand their structure and function.
  8. Explain the concepts of cooperativity as they apply to the function of multimeric proteins.
  9. Explain the ways in which enzymes are able to catalyze reactions.
  10. Explain how enzyme kinetics can be used to understand the mechanisms of enzyme catalysis and enzyme inhibition.

Online Quizzes (5): 10%

Midterm test (mid October): 20%

Midterm test (early November):20%

Final Exam: 50%

3 hours in class/week

Lehninger. Principles of Biochemistry 7th Edition, Wiley, 2017.

This text is sold in a traditional textbook format, in a loose-leaf format, or as an E-book. The latter two formats are significantly cheaper. A custom version of the textbook is also available from the bookstore.

The text is required. We will recommend readings from the textbook to be read before coming to class. There are also useful practice questions after each chapter.

For a full understanding of this course, it is essential that you should read the relevant sections of Lehninger, or of any other standard Biochemistry textbook.

Textbooks on Reserve in Bracken Library

Lehninger Principles of Biochemistry, 5th Edition (Freeman, 2008)

Voet & Voet Biochemistry, 4th Edition (Wiley, 2011)

Garrett & Grisham Biochemistry, 4th Edition (Saunders, 2010)

Queen’s Official Grade Conversion Scale

Grade  Numerical Course Average (Range)

A+            90-100

A              85-89

A-             80-84

B+            77-79

B              73-76

B-             70-72

C+            67-69

C              63-66

C-             60-62

D+            57-59

D              53-56

D-             50-52

F              49 and below